Recombinant Rat C->U-editing enzyme APOBEC-1 (Apobec1)

Code CSB-YP001919RA
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP001919RA
MSDS
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Source E.coli
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Code CSB-EP001919RA-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP001919RA
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP001919RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Apobec1
Uniprot No.
Alternative Names
Apobec1C->U-editing enzyme APOBEC-1; EC 3.5.4.36; Apolipoprotein B mRNA-editing enzyme 1; mRNA(cytosine(6666)) deaminase 1
Species
Rattus norvegicus (Rat)
Expression Region
1-229
Target Protein Sequence
MSSETGPVAV DPTLRRRIEP HEFEVFFDPR ELRKETCLLY EINWGGRHSI WRHTSQNTNK HVEVNFIEKF TTERYFCPNT RCSITWFLSW SPCGECSRAI TEFLSRYPHV TLFIYIARLY HHADPRNRQG LRDLISSGVT IQIMTEQESG YCWRNFVNYS PSNEAHWPRY PHLWVRLYVL ELYCIILGLP PCLNILRRKQ PQLTFFTIAL QSCHYQRLPP HILWATGLK
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Catalytic component of the apolipoprotein B mRNA editing enzyme complex which is responsible for the postranscriptional editing of a CAA codon for Gln to a UAA codon for stop in the APOB mRNA. May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation.
Gene References into Functions
  1. To overcome this limitation, we developed a CRISPR-Cpf1-based BE by fusing the rat cytosine deaminase APOBEC1 to a catalytically inactive version of Lachnospiraceae bacterium Cpf1. PMID: 29553573
  2. Results show that expression of APOBEC1 induces a mutator phenotype in 2 different cellular models. PMID: 25085003
  3. Apobec-1 increased COX-2 expression and COX-2 mRNA stabilization in neurogenic cells, and failed to catalyze C-to-U editing of COX-2 mRNA PMID: 23609497
  4. It was found that rat APOBEC-1 overexpression had a hypermutation pattern similar to that of APOBEC-3s on its substrate apolipoprotein B mRNA. PMID: 22326345
  5. Taken together, these results demonstrate a model in which APOBEC1 induction during encephalitis in neurons may aid in thwarting HSV-1 infection. PMID: 21775448
  6. observations allow speculation (i) that APOBEC-mediated C-to-U editing may contribute to the sequence variation of viruses that replicate entirely through RNA, and (ii) that additional cellular RNA substrates might exist for the APOBEC enzymes PMID: 15286366
  7. may have roles in epigenetic reprogramming and cell plasticity PMID: 15448152
  8. Editing by APOBEC-1 was studied in yeast using Gal4 linked to Gal80 via an apo B cassette (Gal4-C) or via a mouse Ig heavy chain gene variable region (Gal4-VH). APOBEC-1 induced C to U editing in ~15% of the Gal4-C transcripts, but did not affect Gal4-VH. PMID: 15963568

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Subcellular Location
Cytoplasm.
Protein Families
Cytidine and deoxycytidylate deaminase family
Tissue Specificity
Expressed in the liver as well as small intestine.
Database Links
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